DNA Profiling
DNA profiling has revolutionized biological identification and forensic science. The principles we covered in the last chapter such as electrophoresis, genetic marker systems and combining probabilities used in a more expansive and specific way. The material covered in this chapter is also more complex than we have tackled so far.
We’ll begin with a brief discussion of early DNA methods and move quickly into current methods and practices. Topics include short tandem repeats (STRs), amplification of DNA using PCR, and instrumental analysis via capillary electrophoresis. You will the basics of how to interpret a DNA analysis report, and how frequency databases are used to generate probabilistic metrics regarding the output.
Learning Objectives
10.1 Describe the sources and basic structure of DNA
10.2 Differentiate between genes, loci, VNTRs, and STRs
10.3 Describe the basics of PCR amplification of STRs including the use and function of labeled primers
10.4 Recognize key features of an electropherogram and their functions
10.5 Describe the steps of a DNA profiling analysis
10.6 Describe how DNA results are interpreted
10.7 Recognize the application of combined probabilities and frequency databases in DNA profiling.
